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Message definition (Amino acid ) For amino acids with uncharged side-chains the zwitterion predominates at pH values between the two p''K''<sub>a</sub> values, but coexists in [[Chemical equilibrium|equilibrium]] with small amounts of net negative and net positive ions. At the midpoint between the two p''K''<sub>a</sub> values, the trace amount of net negative and trace of net positive ions balance, so that average net charge of all forms present is zero. This pH is known as the [[isoelectric point]] p''I'', so p''I'' = {{sfrac|1|2}}(p''K''<sub>a1</sub> + p''K''<sub>a2</sub>).
荷電していない側鎖を持つアミノ酸の場合、2つのpK a 値の間のpH値では双性イオンが優勢であるが、少量の正負イオンと平衡 で共存する。2つのpK a 値の中間点では、微量の正負イオンと微量の正イオンが釣り合い、存在するすべての形態の平均正電荷はゼロになる。このpHは等電点p I と呼ばれ、pI =1 / 2 K a1 + pK a2 )となる。