All translations
Enter a message name below to show all available translations.
Found 2 translations.
| Name | Current message text |
|---|---|
| h English (en) | ==Synthesis== ===Biosynthesis=== {{Main|Cobalamin biosynthesis}} Vitamin B<sub>12</sub> is derived from a [[tetrapyrrole|tetrapyrrolic structural framework]] created by the enzymes [[Porphobilinogen deaminase|deaminase]] and [[uroporphyrinogen III synthase|cosynthetase]] which transform [[aminolevulinic acid]] via [[porphobilinogen]] and [[hydroxymethylbilane]] to [[uroporphyrinogen III]]. The latter is the first [[macrocycle|macrocyclic]] intermediate common to [[heme]], [[chlorophyll]], [[siroheme]] and B<sub>12</sub> itself. Later steps, especially the incorporation of the additional methyl groups of its structure, were investigated using <sup>13</sup>C [[isotopic labelling|methyl-labelled]] [[S-adenosyl methionine]]. It was not until a [[genetic engineering|genetically engineered]] strain of ''[[Pseudomonas denitrificans]]'' was used, in which eight of the genes involved in the biosynthesis of the vitamin had been [[gene expression|overexpressed]], that the complete sequence of [[methylation]] and other steps could be determined, thus fully establishing all the intermediates in the pathway. |
| h Japanese (ja) | ==合成== {{Anchor|Synthesis}} ===生合成=== {{Main/ja|Cobalamin biosynthesis/ja}} ビタミンB<sub>12</sub>は、[[porphobilinogen/ja|ポルホビリノーゲン]]と[[hydroxymethylbilane/ja|ヒドロキシメチルビラン]]を介して[[aminolevulinic acid/ja|アミノレブリン酸]]を[[uroporphyrinogen III/ja|ウロポルフィリノーゲンIII]]に変換する酵素[[Porphobilinogen deaminase/ja|デアミナーゼ]]と[[uroporphyrinogen III synthase/ja|コシンテターゼ]]によって作られる[[tetrapyrrole/ja|テトラピロール構造骨格]]に由来する。後者は[[heme/ja|ヘム]]、[[chlorophyll/ja|クロロフィル]]、[[siroheme/ja|シロヘム]]およびB<sub>12</sub>そのものに共通する最初の[[macrocycle/ja|マクロ環状]]中間体である。後の段階、特にその構造の付加的なメチル基の組み込みは、<sup>13</sup>C [[isotopic labelling/ja|メチル標識]][[S-adenosyl methionine/ja|S-アデノシルメチオニン]]を用いて研究された。。ビタミンの生合成に関与する8つの遺伝子が[[gene expression/ja|過剰発現]]された''[[Pseudomonas denitrificans/ja|シュードモナス・デニトリフィカンス]]''の[[genetic engineering/ja|遺伝子操作]]株が使用されるまでは、[[メチル化]]および他のステップの完全な配列が決定されることはなかった。 |