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 h English (en){{chem|B|12r}} and {{chem|B|12s}} can be prepared from cyanocobalamin by controlled potential reduction, or chemical reduction using [[sodium borohydride]] in alkaline solution, [[zinc]] in [[acetic acid]], or by the action of [[thiols]]. Both {{chem|B|12r}} and {{chem|B|12s}} are stable indefinitely under oxygen-free conditions. {{chem|B|12r}} appears orange-brown in solution, while {{chem|B|12s}} appears bluish-green under natural daylight, and purple under artificial light.
 h Japanese (ja){{chem|B|12r}}と{{chem|B|12s}}はシアノコバラミンから電位制御還元、またはアルカリ溶液中の[[sodium borohydride/ja|水素化ホウ素ナトリウム]]、[[acetic acid/ja|酢酸]]中の[[zinc/ja|亜鉛]]を用いた化学還元、または[[thiols/ja|チオール]]の作用によって調製することができる。{{chem|B|12r}}も{{chem|B|12s}}も酸素のない条件下では無期限に安定である。{{chem|B|12r}}は溶液中では橙褐色に、{{chem|B|12s}}は自然光の下では青緑色に、人工光の下では紫色に見える。