Translations:Flavin adenine dinucleotide/30/en

Prenylcysteine lyase (PCLase) catalyzes the cleavage of prenylcysteine (a protein modification) to form an isoprenoid aldehyde and the freed cysteine residue on the protein target. The FAD is non-covalently bound to PCLase. Not many mechanistic studies have been done looking at the reactions of the flavin, but the proposed mechanism is shown below. A hydride transfer from the C1 of the prenyl moiety to FAD is proposed, resulting in the reduction of the flavin to FADH₂. COformED IS a carbocation that is stabilized by the neighboring sulfur atom. FADH₂ then reacts with molecular oxygen to restore the oxidized enzyme.