https://wiki.tiffa.net/w/index.php?action=history&feed=atom&title=Translations%3AProtein%2F59%2FenTranslations:Protein/59/en - Revision history2026-04-20T14:23:35ZRevision history for this page on the wikiMediaWiki 1.43.0https://wiki.tiffa.net/w/index.php?title=Translations:Protein/59/en&diff=119988&oldid=prevFuzzyBot: Importing a new version from external source2024-02-23T07:36:06Z<p>Importing a new version from external source</p>
<p><b>New page</b></p><div>For natural proteins, a series of purification steps may be necessary to obtain protein sufficiently pure for laboratory applications. To simplify this process, [[genetic engineering]] is often used to add chemical features to proteins that make them easier to purify without affecting their structure or activity. Here, a "tag" consisting of a specific amino acid sequence, often a series of [[histidine]] residues (a "[[His-tag]]"), is attached to one terminus of the protein. As a result, when the lysate is passed over a chromatography column containing [[nickel]], the histidine residues ligate the nickel and attach to the column while the untagged components of the lysate pass unimpeded. A number of different tags have been developed to help researchers purify specific proteins from complex mixtures.</div>FuzzyBot