https://wiki.tiffa.net/w/index.php?action=history&feed=atom&title=Translations%3AInsulin%2F77%2Fen 2026-09-14T13:24:29Z Revision history for this page on the wiki MediaWiki 1.43.0 https://wiki.tiffa.net/w/index.php?title=Translations:Insulin/77/en&diff=129931&oldid=prev 2024-03-18T07:33:32Z

<p>Importing a new version from external source</p> <p><b>New page</b></p><div>Recombinant insulin is produced either in yeast (usually &#039;&#039;[[baker&#039;s yeast|Saccharomyces cerevisiae]]&#039;&#039;) or &#039;&#039;E. coli&#039;&#039;. In yeast, insulin may be engineered as a single-chain protein with a KexII endoprotease (a yeast homolog of PCI/PCII) site that separates the insulin A chain from a C-terminally truncated insulin B chain. A chemically synthesized C-terminal tail is then grafted onto insulin by reverse proteolysis using the inexpensive protease trypsin; typically the lysine on the C-terminal tail is protected with a chemical protecting group to prevent proteolysis. The ease of modular synthesis and the relative safety of modifications in that region accounts for common insulin analogs with C-terminal modifications (e.g. lispro, aspart, glulisine). The Genentech synthesis and completely chemical synthesis such as that by [[Bruce Merrifield]] are not preferred because the efficiency of recombining the two insulin chains is low, primarily due to competition with the precipitation of insulin B chain.</div>

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