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{| class="wikitable" style="text-align:center;"
|-
! colspan="3" style="background:orange; color:black;" | I. Chemical methods
|-
! colspan="3" style="background:lightblue; color:black;" | A. Oxidation-reduction reaction
|-
| colspan="3" style="background-color:white; color:black;" | <math>\mathrm{Glucose} + \mathrm{Alkaline\ copper\ tartarate}\xrightarrow{\mathrm{Reduction}} \mathrm{Cuprous\ oxide} </math>
|-
! width="900pt" colspan="3" | 1. Alkaline copper reduction
|-
| Folin-Wu method
| style="background-color:white; color:black;" | <math>\mathrm{Cu}^{2+} + \mathrm{Phosphomolybdic\ acid}\xrightarrow{\mathrm{Oxidation}} \mathrm{Phosphomolybdenum\ oxide}</math>
| Blue end-product
|-
| Benedict's method
| colspan="2" style="background-color:white; color:black;" |
* Modification of Folin–Wu method for qualitative urine glucose.
|-
| Nelson–Somogyi method
| style="background-color:white; color:black;" | <math>\mathrm{Cu}^{2+} + \mathrm{Arsenomolybdic\ acid}\xrightarrow{\mathrm{Oxidation}} \mathrm{Arsenomolybdenum\ oxide}</math>
| Blue end-product.
|-
| Neocuproine method
| style="background-color:white; color:black;" | <math>\mathrm{Cu}^{2+} + \mathrm{Neocuproine}\xrightarrow{\mathrm{Oxidation}} \mathrm{Cu}^{2+} \mathrm{neocuproine\ complex} </math>*
| Yellow-orange color neocuproine
|-
| Shaeffer–Hartmann–Somogyi
| style="background-color:white; color:black;" |
* Uses the principle of [[iodine]] reaction with cuprous byproduct.
* Excess I<sub>2</sub> is then titrated with [[thiosulfate]].
|-
! colspan="3" | 2. Alkaline Ferricyanide reduction
|-
| Hagedorn–Jensen
| style="background-color:white; color:black;" | <math>\mathrm{Glucose} + \mathrm{Alkaline\ ferricyanide}\longrightarrow \mathrm{Ferrocyanide}</math>
| Colorless end product; other reducing substances interfere with reaction.
|-
| colspan="3" style="background:lightblue; color:black;" | B. Condensation
|-
| Ortho-toluidine method
| colspan="2" style="background-color:white; color:black;" |
* Uses [[aromatic amine]]s and hot [[acetic acid]].
* Forms [[glycosylamine]] and Schiff's base which is emerald green in color.
* This is the most specific method, but the reagent used is toxic.
|-
| Anthrone (phenols) method
| colspan="2" style="background-color:white; color:black;" |
* Forms hydroxymethyl furfural in hot acetic acid
|-
! colspan="3" style="background:orange; color:black;" | II. Enzymatic methods
|-
! colspan="3" style="background:lightblue; color:black;" | A. Glucose oxidase
|-
| colspan="3" style="background-color:white; color:black;" |<math>\mathrm{Glucose} + \mathrm{O}_{2}\xrightarrow[\mathrm{Oxidation}] {\mathrm{glucose\ oxidase}}\textrm{D-glucono-1,5-lactone} + \mathrm{H_{2}O_{2}} </math>
|-
| Saifer–Gerstenfeld method
| style="background-color:white; color:black;" | <math>\mathrm{H_{2}O_2} + \textit{O}\text{-dianisidine}\xrightarrow[\mathrm{Oxidation}] {\mathrm{peroxidase}} \mathrm{H_2O} + \mathrm{oxidized\ chromogen}</math>
| Inhibited by reducing substances like BUA, [[bilirubin]], [[glutathione]], [[ascorbic acid]].
|-
| Trinder method
| colspan="2" style="background-color:white; color:black;" |
* Uses [https://web.archive.org/web/20061214205244/http://www.online-medical-dictionary.org/4-Aminophenazone.asp?q=4-Aminophenazone 4-aminophenazone] oxidatively coupled with phenol.
* Subject to less interference by increases serum levels of [[creatinine]], [[uric acid]] or [[hemoglobin]].
* Inhibited by [[catalase]].
|-
| Kodak Ektachem
| colspan="2" style="background-color:white; color:black;" |
* A dry chemistry method.
* Uses [[spectrophotometry]] to measure the intensity of color through a lower transparent film.
|-
| Glucometer
| colspan="2" style="background-color:white; color:black;" |
* Home monitoring blood glucose assay method.
* Uses a strip impregnated with a glucose oxidase reagent.
|-
! colspan="3" style="background:lightblue; color:black;" | B. Hexokinase
|-
| colspan="3" style="background-color:white; color:black;" |
<math>\begin{alignat}{2}
& \mathrm{Glucose} + \mathrm{ATP}\xrightarrow[\mathrm{Phosphorylation}] {\mathrm{Hexokinase} + \mathrm{Mg}^{2+}} \textrm{G-6PO}_4 + \mathrm{ADP} \\
& \textrm{G-6PO}_4 + \mathrm{NADP}\xrightarrow[\mathrm{Oxidation}] {\textrm{G-6PD}} \textrm{6-Phosphogluconate} + \mathrm{NADPH} + \mathrm{H}^{+} \\
\end{alignat}</math>
|-
| colspan="3" style="background-color:white; color:black;" |
* [[NADP]] as cofactor.
* NADPH (reduced product) is measured in 340 nm.
* More specific than glucose oxidase method due to G-6PO<sub>4</sub>, which inhibits interfering substances except when sample is hemolyzed.
|}

Translations:Blood sugar level/39/en - Revision history

FuzzyBot: Importing a new version from external source